The current method describes the derivative spectrophotometry with absorbance correction method for the estimation of tramadol hydrochloride (TRM), diclofenac sodium (DIC) and chlorzoxazone (CHL) from their combined tablet dosage form by using 0.1 N NaOH as a solvent. The first derivative absorption at 243.75 nm, 265.08 nm and 272.25 nm were used for the estimation of diclofenac sodium, tramadol hydrochloride and chlorzoxazone. The method follows beer’s linearity in the range of 5-45 µg/ml for tramadol hydrochloride, 10-50 µg/ml for diclofenac sodium and 10-50 µg/ml for chlorzoxazone. The % recovery was found to be 99.33 - 99.77 % for TRM, 99.5 – 100.3% for DIC and 99.63 - 99.79 % for CHL. The proposed method has been validated as per ICH guidelines and successfully applied to the estimation of TRM, DIC and CHL from their combined Tablet dosage form.
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